Talks aimed at resolving the Southern Railway dispute have ended without agreement, the company said.
Aslef members are set to go on strike again on Friday in the third day of industrial action this week.
The long-running saga is over union opposition to driver-only trains, with concerns passenger safety will be compromised if drivers are responsible for closing doors using in-cab monitors.
Talks will be held tomorrow in a bid to try and resolve the Southern Railway drivers' dispute.
It comes after the first day of 48-hour walkout by members of Aslef left 300,000 people with no way of getting to work.
Tomorrow's strike is still set to go ahead, as well as another 24-hour stoppage on Friday.
Southern Railway services will be at a standstill again on Friday after talks over driver-only trains collapsed.
Talks aimed at resolving the Southern Railway drivers' dispute will be held at the conciliation service Acas.
Commuters have been taking to social media to express their frustration at the latest Southern Rail strike.