Talks aimed at resolving the Southern Railway dispute have ended without agreement, the company said.
Aslef members are set to go on strike again on Friday in the third day of industrial action this week.
The long-running saga is over union opposition to driver-only trains, with concerns passenger safety will be compromised if drivers are responsible for closing doors using in-cab monitors.
A single mother who fears she will lose her job because of delays commuting to work during the Southern rail strike has written an emotional message to her local MP.
Jenny Lehane said she was writing the email with "tears streaming down my face", as she pleaded with the government to "do anything" to stop the industrial action.
In the message, also posted on the ITV News Facebook page, Ms Lehane said she had been forced to get her six-year-old son out of bed at 5.30am to take him to school on "another three hour journey".
She said: "Do you have any idea of the stress, worry and upset this is causing. I HAVE to go to work. I am a single parent and my 6 year old needs his mother to work to keep a roof over our heads.
"A message from me to all involved: hang your heads in shame!"
Southern Railway services will be at a standstill again on Friday after talks over driver-only trains collapsed.
Talks aimed at resolving the Southern Railway drivers' dispute will be held at the conciliation service Acas.
Commuters have been taking to social media to express their frustration at the latest Southern Rail strike.