Forty-five migrants were taken by ferry from Lesbos to Turkey as Greek authorities restarted returns under an EU deal to stem the flow of migrants and refugees to Europe.
The ferry left the port of Mytilene on Friday morning, with a second boat expected to leave later, state television reported.
The first group of 202 migrants to be returned, most of them from Pakistan and Afghanistan, were taken back to Turkey on Monday.
Several hundred migrants have been pictured staging a sit down protest at a detention camp on the island of Lesbos.
Europe Editor James Mates reports that protesters shouted "I want freedom".
The Democratic presidential candidate may also have shown his cards on his choice of running mate.
The US president also shared a post on Twitter accusing Dr Anthony Fauci of misleading the public over hydroxychloroquine.
Fears over an impending second wave of coronavirus dominates Wednesday’s front pages.